Váradi K, Elödi S
Thromb Haemost. 1976 Jun 30;35(3):576-85.
Human factor IXa was purified 5,000-fold from serum by ion exchange chromatography. The preparation was free from other clotting factors. Both pH sensitivity and heat stability of purified factor IXa appeared to be different from those of factor IX in the plasma. The molecular weight of human factor IXa is 80,000 as estimated from gel-filtration experiments. Modification of seryl or histidyl side chains abolished the activity of factor IXa.
人凝血因子IXa通过离子交换色谱法从血清中纯化了5000倍。该制剂不含其他凝血因子。纯化的因子IXa的pH敏感性和热稳定性似乎与血浆中的因子IX不同。根据凝胶过滤实验估计，人凝血因子IXa的分子量为80,000。丝氨酰或组氨酰侧链的修饰使因子IXa的活性丧失。
