3'-端带有d(GCGAAGC)发夹结构的寡脱氧核糖核苷酸的杂交动力学

Hybridization kinetics of oligodeoxyribonucleotides with a d(GCGAAGC) hairpin at the 3'-end.

作者信息

Chraïbi Z, Réfrégiers M, Jollès B, Laigle A

机构信息

Laboratoire d'immunologie et endocrinologie, Université Cadi Ayyad, Marrakech, Morocco.

出版信息

J Biomol Struct Dyn. 1999 Dec;17(3):539-44. doi: 10.1080/07391102.1999.10508384.

DOI:10.1080/07391102.1999.10508384

PMID:10636088

Abstract

In order to protect them against enzymatic attack of serum in the antisense strategy, oligodeoxyribonucleotides can be protected on their 3'-side by the sequence d(GCGAAGC) which spontaneously forms a hairpin which is known for its extraordinary stability with regard to thermal denaturation or nuclease degradation (I. Hirao, G. Kawai, S. Yoshizawa, Y. Nishimura, Y. Ishido, K. Watanabe and K. Miura, Nucleic Acids Res. 22, 576-582 (1994)). By contrast, the hairpin does not prevent hybridization of the 5'-stem part of the oligonucleotide to a target DNA strand. As soon as this pairing occurs, the stability of the hairpin is disrupted. Its opening rate, followed by its pairing if possible, is of the same order than that of hybridization of the stem part.

摘要

为了在反义策略中保护它们免受血清中酶的攻击，寡脱氧核糖核苷酸可以在其3'端被序列d(GCGAAGC)保护，该序列会自发形成一个发夹结构，该发夹结构因其在热变性或核酸酶降解方面具有非凡的稳定性而闻名（I. 平尾、G. 川合、S. 吉泽、Y. 西村、Y. 石户、K. 渡边和K. 三浦，《核酸研究》22，576 - 582(1994)）。相比之下，该发夹结构并不妨碍寡核苷酸5'端茎部与靶DNA链的杂交。一旦发生这种配对，发夹结构的稳定性就会被破坏。其打开速率，随后如果可能的话其配对速率，与茎部杂交的速率处于同一量级。