异常稳定的微型发夹d(GCGAAGC)的打开。
Opening of the extraordinarily stable mini-hairpin d(GCGAAGC).
作者信息
Jollès B, Réfrégiers M, Laigle A
机构信息
LPBC (CNRS URA 2056), Université Pierre et Marie Curie, 4 Place Jussieu, Case 138, 75252 Paris Cedex 05, France.
出版信息
Nucleic Acids Res. 1997 Nov 15;25(22):4608-13. doi: 10.1093/nar/25.22.4608.
Abstract
For the purposes of the antisense strategy oligodeoxyribonucleotides can be protected against serum and cell nuclease digestion by tagging at their 3'-end with a sequence naturally forming a very stable hairpin, d(GCGAAGC). This nuclease-resistant hairpin is also known for its high thermostability. We demonstrate in this study that attachment of d(GCGAAGC) at the 3'-end of an oligodeoxyribonucleotide does not hinder hybridization of the 5'-part of this oligonucleotide to a complementary DNA strand. Moreover, the hairpin is in equilibrium between a folded and an open structure, with an energy minimum in favor of pairing if it is possible, even with mismatches.
摘要
为了反义策略的目的，寡脱氧核糖核苷酸可以通过在其3'-末端标记一个天然形成非常稳定发夹结构的序列d(GCGAAGC)来防止血清和细胞核酸酶的消化。这种抗核酸酶的发夹结构也因其高耐热性而闻名。我们在本研究中证明，在寡脱氧核糖核苷酸的3'-末端连接d(GCGAAGC)不会阻碍该寡核苷酸5'-部分与互补DNA链的杂交。此外，发夹结构在折叠和开放结构之间处于平衡状态，如果可能的话，即使存在错配，其能量最小值也有利于配对。
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本文引用的文献
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