Knoll A, Sargueil F, Salles J, Cassagne C, Garbay B
Laboratoire de Biogenèse Membranaire, CNRS-UMR 5544, Université Victor Segalen Bordeaux 2, 146, Rue Léo Saignat Case 92, 33076, Bordeaux, France.
Brain Res Mol Brain Res. 1999 Dec 10;74(1-2):217-20. doi: 10.1016/s0169-328x(99)00252-1.
Despite increasing insight into peroxisomal beta-oxidation, it is still not clear which enzymes catalyze very-long-chain fatty acid degradation. Using the northern blot and RT-PCR techniques, a brain-specific expression is demonstrated for acyl-CoA oxidase 3II mRNA, thiolase-A and trans2,3enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase multifunctional enzyme type 2.
尽管对过氧化物酶体β-氧化的认识不断深入，但仍不清楚哪些酶催化极长链脂肪酸的降解。利用Northern印迹法和RT-PCR技术，证明了酰基辅酶A氧化酶3II mRNA、硫解酶-A和2,3-反式烯酰辅酶A水合酶/3-羟基酰基辅酶A脱氢酶多功能酶2在脑中特异性表达。
