Hanamura A, Kinoshita T, Kurokawa T, Nagai H, Murate T, Nagasaka T, Mori H, Saito H
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Int J Hematol. 1999 Dec;70(4):283-9.
Bone marrow (BM) involvement in peripheral T-cell lymphoma was assessed by polymerase chain reaction (PCR)-mediated RNase protection assay. The sensitivity of this assay was approximately 10(-4) to 10(-5). In 16 of 30 patients (53.3%) with peripheral T-cell malignancies, consensus primers for the T-cell receptor (TCR)-gamma gene amplified the rearranged V(N)J region. Using the PCR products of diagnostic lymph nodes of the patients as probes, we analyzed the BM involvement of lymphoma cells in eight patients: four with peripheral T-cell lymphoma, unspecified; two with adult T-cell leukemia/lymphoma; and two with angioimmunoblastic T-cell lymphoma. BM involvement was detected by PCR-mediated RNase protection assay in four patients from BM smear and/or histo-pathological examination of clotted BM. Moreover, in two of four patients in whom BM involvement was not evident from morphological examination, BM involvement was detected by PCR-mediated RNase protection assay. Our results indicate that the PCR-mediated RNase protection assay targeting the TCR-gamma gene is useful in detecting minimal residual disease in about half of all T-cell lymphoma cases. In addition, in some patients with peripheral T-cell lymphoma, morphologically unproven BM involvement was found using the method.
采用聚合酶链反应（PCR）介导的核糖核酸酶保护分析评估外周T细胞淋巴瘤患者的骨髓（BM）受累情况。该分析的灵敏度约为10^(-4)至10^(-5)。在30例外周T细胞恶性肿瘤患者中的16例（53.3%）中，T细胞受体（TCR）-γ基因的共有引物扩增出重排的V(N)J区域。以患者诊断性淋巴结的PCR产物为探针，我们分析了8例患者淋巴瘤细胞的骨髓受累情况：4例为未特定的外周T细胞淋巴瘤；2例为成人T细胞白血病/淋巴瘤；2例为血管免疫母细胞性T细胞淋巴瘤。通过PCR介导的核糖核酸酶保护分析，在4例患者的骨髓涂片和/或凝块骨髓组织病理学检查中检测到骨髓受累。此外，在4例经形态学检查未发现明显骨髓受累的患者中，有2例通过PCR介导的核糖核酸酶保护分析检测到骨髓受累。我们的结果表明，针对TCR-γ基因的PCR介导的核糖核酸酶保护分析在检测约一半的所有T细胞淋巴瘤病例中的微小残留病方面是有用的。此外，使用该方法在一些外周T细胞淋巴瘤患者中发现了形态学上未证实的骨髓受累情况。
