Konet D S, Mezencio J M, Babcock G, Brown F
Plum Island Animal Disease Center, Greenport, NY 11944, USA.
J Virol Methods. 2000 Jan;84(1):95-8. doi: 10.1016/s0166-0934(99)00131-7.
Amplification by RT-PCR of the RNA present in foot-and-mouth disease virus particles is inhibited by substances present in the sera of several species. This inhibition appears to be caused by a direct interaction of the substances with the RNA and not the enzymes used for its amplification.
口蹄疫病毒颗粒中存在的RNA通过RT-PCR进行扩增时,会受到几种物种血清中存在的物质的抑制。这种抑制似乎是由这些物质与RNA直接相互作用引起的,而非与其扩增所用的酶发生相互作用所致。