人白血病成髓细胞中核DNA聚合酶的评估

Nuclear DNA-polymerase estimation in human leukaemic myeloblasts.

作者信息

Wantzin G L, Karle H, Killmann S A

出版信息

Br J Haematol. 1976 Jul;33(3):329-34. doi: 10.1111/j.1365-2141.1976.tb03548.x.

DOI:10.1111/j.1365-2141.1976.tb03548.x

Abstract

A technique which detects the presence of DNA-polymerase in cell nuclei by measuring the incorporation of 3H-thymidine-5-triphosphate (3H-TTP) has been used to estimate the proportion of leukaemic myeloblasts which contains DNA-polymerase and DNA capable of acting as primer-template. In six cases of previously untreated acute myeloid leukaemia the 3H-TTP labelling index (3H-TTP LI) was much larger than the fraction in DNA synthesis. After a single 'flash' injection of cytosine arabinoside a pronounced decline was observed in the 3H-TTP LI, which can be explained by a direct inhibition of NDA-polymerase. No change was observed in the fraction of cells labelled with 3H-thymidine. A decrease in 3H-TTP LI was also observed after a single i.v. dose of methotrexate.

摘要

一种通过测量3H-胸苷-5-三磷酸（3H-TTP）的掺入来检测细胞核中DNA聚合酶存在的技术，已被用于估计含有DNA聚合酶和能够作为引物模板的DNA的白血病成髓细胞的比例。在6例未经治疗的急性髓性白血病病例中，3H-TTP标记指数（3H-TTP LI）远大于DNA合成中的比例。单次“快速”注射阿糖胞苷后，观察到3H-TTP LI显著下降，这可以通过对NDA聚合酶的直接抑制来解释。用3H-胸苷标记的细胞比例未观察到变化。单次静脉注射甲氨蝶呤后也观察到3H-TTP LI下降。