Quinn C M, Wiles A P, El-Shanawany T, Catchpole I, Alnadaf T, Ford M J, Gordon S, Greaves D R
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, United Kingdom.
Genomics. 1999 Dec 15;62(3):468-76. doi: 10.1006/geno.1999.6031.
The gene encoding human eukaryotic initiation factor 4A (EIF4A1) is located on chromosome 17p13, 667 bp upstream from the gene encoding the macrophage endosomal protein CD68. The EIF4AI gene contains 10 intervening sequences with the 1397-bp first intron containing a CpG-rich methylation-free island. Sequences capable of enhancing gene expression reside between positions -69 and -371 and positions -504 and -1100 of the EIF4AI 5' flanking sequence and within introns 1, 2, 3, 7, and 9. In macrophage cell lines, EIF4A1 expression vectors give sustained high-level reporter gene expression to levels 10 times higher than that obtained using the human cytomegalovirus immediate-early gene promoter/enhancer. Sequences of the human EIF4AI gene may find application in the development of new vectors for gene therapy and genetic vaccination.
编码人类真核生物起始因子4A（EIF4A1）的基因位于17号染色体p13上，距离编码巨噬细胞内体蛋白CD68的基因上游667 bp处。EIF4AI基因包含10个间隔序列，其中1397 bp的第一个内含子含有一个富含CpG的无甲基化岛。能够增强基因表达的序列位于EIF4AI 5'侧翼序列的-69至-371位和-504至-1100位之间以及内含子1、2、3、7和9内。在巨噬细胞系中，EIF4A1表达载体可使报告基因持续高水平表达，其表达水平比使用人巨细胞病毒立即早期基因启动子/增强子获得的水平高10倍。人类EIF4AI基因的序列可能在基因治疗和基因疫苗新载体的开发中得到应用。
