Keough R, Woollatt E, Crawford J, Sutherland G R, Plummer S, Casey G, Gonda T J
Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, South Australia, 5000, Australia.
Genomics. 1999 Dec 15;62(3):483-9. doi: 10.1006/geno.1999.6035.
We have previously isolated and characterized murine MYB binding protein (p160) 1a, a protein that specifically interacts with the leucine zipper motif within the negative regulatory domain of the c-Myb proto-oncoprotein. We now describe the molecular cloning of the human MYBBP1A cDNA and chromosomal localization to 17p13.3 by fluorescence in situ hybridization analysis. Given the likely presence of a tumor suppressor gene (or genes) within this region of chromosome 17, the position of MYBBP1A was further mapped by radiation hybrid analysis and was found to lie between markers D17S1828 and D17S938. A P1 artificial chromosome clone containing the 5' region of MYBBP1A was isolated and indicates a physical linkage between MYBBP1A and the 15-lipoxygenase gene (ALOX15). A novel, polymorphic (CA)(25) dinucleotide repeat was also isolated from this PAC and may serve as a useful marker for MYBBP1A and this region of chromosome 17.
我们之前已经分离并鉴定了小鼠MYB结合蛋白（p160）1a，该蛋白能与c-Myb原癌蛋白负调控域内的亮氨酸拉链基序特异性相互作用。我们现在描述人类MYBBP1A cDNA的分子克隆以及通过荧光原位杂交分析将其染色体定位到17p13.3。鉴于17号染色体该区域可能存在一个或多个肿瘤抑制基因，通过辐射杂种分析对MYBBP1A的位置进行了进一步定位，发现它位于标记D17S1828和D17S938之间。分离出了一个包含MYBBP1A 5'区域的P1人工染色体克隆，表明MYBBP1A与15-脂氧合酶基因（ALOX15）之间存在物理连锁。还从该PAC中分离出了一个新的多态性（CA）(25)二核苷酸重复序列，它可能作为MYBBP1A和17号染色体该区域的有用标记。
