Ueda K, Nozawa M, Nakao M, Miyasaka M, Byun S I, Tajima S
Department of Plastic and Reconstructive Surgery, Osaka Medical College, Takatsuki, Osaka, 569-8686, Japan.
J Surg Res. 2000 Feb;88(2):125-9. doi: 10.1006/jsre.1999.5785.
Sulfatide binds to P- and L-selectin, which play important roles in the initiation of neutrophil-endothelial interactions. Sulfatide protects skin flaps from ischemia-reperfusion injury. The purpose of this study was to evaluate the augmented protection when anti-rat ICAM-1 and anti-rat LFA-1 antibodies are combined with sulfatide in the ischemia-reperfusion model of rat skin flaps. Sulfatide was administered intravenously just before elevation of the right abdominal epigastric flap, and monoclonal antibodies were injected 30 min before clamp release. The femoral artery and vein were clamped above and below the epigastric vessels for 11 h and then the clamp was released. The administration of both sulfatide and monoclonal antibodies significantly increased the flap surviving area (6.58 +/- 0.61 cm(2) versus the group with monoclonal antibodies alone, 4.43 +/- 0.32 cm(2), P = 0.01). In the untreated rats the area was 1.86 +/- 0.36 cm(2). Histological examination 24 h after reperfusion in the group treated with sulfatide and monoclonal antibodies showed only slight leukocyte invasion into the flap, and myeloperoxidase activity 24 h after reperfusion was significantly reduced. This study indicates that both sulfatide and monoclonal antibodies protect rat skin flaps from ischemia-reperfusion injury.
硫脂与P-选择素和L-选择素结合，这两种选择素在中性粒细胞与内皮细胞相互作用的起始过程中发挥重要作用。硫脂可保护皮瓣免受缺血再灌注损伤。本研究的目的是评估在大鼠皮瓣缺血再灌注模型中，抗大鼠ICAM-1和抗大鼠LFA-1抗体与硫脂联合使用时增强的保护作用。在掀起右腹上区皮瓣之前静脉注射硫脂，在松开血管夹前30分钟注射单克隆抗体。在腹壁血管的上方和下方夹闭股动脉和静脉11小时，然后松开血管夹。硫脂和单克隆抗体联合给药显著增加了皮瓣存活面积（6.58±0.61平方厘米，而单独使用单克隆抗体的组为4.43±0.32平方厘米，P = 0.01）。未治疗大鼠的皮瓣存活面积为1.86±0.36平方厘米。在硫脂和单克隆抗体治疗组再灌注24小时后的组织学检查显示，仅有轻微的白细胞浸润到皮瓣中，再灌注24小时后的髓过氧化物酶活性显著降低。本研究表明，硫脂和单克隆抗体均可保护大鼠皮瓣免受缺血再灌注损伤。
