Wu W X, Zhang Q, Unno N, Derks J B, Nathanielsz P W
Department of Biomedical Sciences, Laboratory for Pregnancy and Newborn Research, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401, USA.
Am J Physiol Cell Physiol. 2000 Jan;278(1):C199-206. doi: 10.1152/ajpcell.2000.278.1.C199.
In this study, we characterized the changes in the extracellular matrix proteoglycan decorin in pregnant intrauterine tissues in late gestation and in association with labor and delivery in sheep. In addition, we examined the effects of estradiol and progesterone on regulation of decorin mRNA expression in myometrium from the nonpregnant ovariectomized sheep. Using suppression subtractive hybridization in combination with Northern blot analysis, we identified a significant increase in decorin mRNA in the pregnant sheep myometrium during labor. The abundance of decorin mRNA paralleled myometrial contractility. The increase in decorin mRNA during labor was only demonstrated in the myometrium; no increase was observed in the endometrium or fetal membranes. Estradiol upregulated decorin mRNA and may act as a potential stimulator responsible for the increased decorin in the myometrium during parturition. The ovine decorin cDNA spans 1288 nt, includes 1083 nt of coding sequence predicted to encode a protein of 360 amino acids, 119 nt of 5'-untranslated region (UTR) and 86 nt of 3'-UTR. Over the coding region, the protein shares 79-96% nt sequence identity and 73-94% identity in the deduced amino acid sequence with homologous mammalian sequences. Using cloned decorin cDNA, we observed that the fibroblasts are the predominant cell type in the pregnant sheep myometrium containing decorin mRNA. These data suggest that increased decorin synthesis participates in the matrix changes that may play a role in myometrial activation.
在本研究中，我们对妊娠晚期绵羊子宫内组织中细胞外基质蛋白聚糖核心蛋白聚糖的变化进行了表征，并研究了其与分娩的关系。此外，我们检测了雌二醇和孕酮对未孕去卵巢绵羊子宫肌层中核心蛋白聚糖mRNA表达调控的影响。通过抑制性消减杂交结合Northern印迹分析，我们发现分娩期间妊娠绵羊子宫肌层中核心蛋白聚糖mRNA显著增加。核心蛋白聚糖mRNA的丰度与子宫肌层收缩性平行。分娩期间核心蛋白聚糖mRNA的增加仅在子宫肌层中出现；子宫内膜或胎膜中未观察到增加。雌二醇上调核心蛋白聚糖mRNA，可能是分娩期间子宫肌层中核心蛋白聚糖增加的潜在刺激因素。绵羊核心蛋白聚糖cDNA跨度为1288 nt，包括1083 nt的编码序列，预计编码一个360个氨基酸的蛋白质、119 nt的5'-非翻译区（UTR）和86 nt的3'-UTR。在编码区域，该蛋白与同源哺乳动物序列的核苷酸序列同一性为79-96%，推导氨基酸序列同一性为73-94%。使用克隆的核心蛋白聚糖cDNA，我们观察到成纤维细胞是妊娠绵羊子宫肌层中含有核心蛋白聚糖mRNA的主要细胞类型。这些数据表明，核心蛋白聚糖合成增加参与了可能在子宫肌层激活中起作用的基质变化。
