Elliott S N, Wallace J L, McKnight W, Gall D G, Hardin J A, Olson M, Buret A
Department, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
Am J Physiol Gastrointest Liver Physiol. 2000 Jan;278(1):G105-12. doi: 10.1152/ajpgi.2000.278.1.G105.
Experimental gastric ulcers are rapidly colonized by various bacteria, resulting in significantly impaired healing. Epidermal growth factor (EGF) is capable of preventing bacterial colonization of the healthy intestinal mucosa. In this study, we examined the possibility that EGF accelerates gastric ulcer healing by reducing bacterial colonization of the ulcer. Gastric ulcers were induced by serosal application of acetic acid. The effect of daily administration of EGF on ulcer healing and bacterial colonization was assessed and compared with the effect of daily treatment with broad-spectrum antibiotics. EGF administration reduced colonization levels and accelerated ulcer healing as effectively as the antibiotic treatment. EGF was without effect on acid secretion or neutrophil infiltration into the ulcer. Bacterial growth was not inhibited in the presence of EGF in vitro. These results demonstrate that EGF reduces bacterial colonization during an established infection of a compromised mucosal surface. This effect may contribute to the ability of EGF to accelerate gastric ulcer healing. This effect is acid independent and not due to an anti-inflammatory effect or to direct bactericidal actions.
实验性胃溃疡会迅速被各种细菌定植，导致愈合显著受损。表皮生长因子（EGF）能够防止细菌定植于健康的肠黏膜。在本研究中，我们探讨了EGF通过减少溃疡处细菌定植来加速胃溃疡愈合的可能性。通过在浆膜面应用醋酸诱导胃溃疡。评估每日给予EGF对溃疡愈合和细菌定植的影响，并与每日使用广谱抗生素治疗的效果进行比较。给予EGF可降低定植水平，并与抗生素治疗一样有效地加速溃疡愈合。EGF对胃酸分泌或中性粒细胞向溃疡处浸润没有影响。在体外，EGF存在时细菌生长未受抑制。这些结果表明，EGF在受损黏膜表面已发生感染期间可减少细菌定植。这种作用可能有助于EGF加速胃溃疡愈合的能力。这种作用不依赖于酸，也不是由于抗炎作用或直接杀菌作用。
