Caruso-Neves C, Monteiro S O, de Oliveira C F, Filho C C, Lopes A G
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Arch Insect Biochem Physiol. 2000 Feb;43(2):72-7. doi: 10.1002/(SICI)1520-6327(200002)43:2<72::AID-ARCH3>3.0.CO;2-E.
The role of adenosine on regulation of the (Na(+)+K(+))ATPase activity present in the Malpighian tubules isolated from Rhodnius prolixus was investigated. Adenosine decreases the (Na(+)+K(+)) ATPase specific activity by 88%, in a dose-dependent manner, with maximal effect at a concentration of 10(-9) M. This effect was mimicked by N(6)-cyclohexyladenosine (CHA) at 10(-8) M, an agonist for A(1) adenosine receptor, and was reversed by 10(-9) M 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), an antagonist for A(1) adenosine receptor. On the other hand, 5'-N-ethyl-carboxamide adenosine (NECA), an agonist for A(2) adenosine receptor, used in the range of 10(-9)-10(-5) M, did not change the (Na(+)+K(+))ATPase specific activity. In the same way, 10(-8) M 3, 7-dimethyl-1-propargylxanthine (DMPX), an antagonist for A(2) adenosine receptor, did not modify the inhibitory effect of adenosine. These data suggest that the inhibitory effect of adenosine on the (Na(+)+K(+))ATPase specific activity present in Malpighian tubules from Rhodnius prolixus is mediated by A(1) adenosine receptor activation. Arch.
研究了腺苷对从长红猎蝽分离的马氏管中存在的（Na⁺+K⁺）ATP酶活性的调节作用。腺苷以剂量依赖性方式使（Na⁺+K⁺）ATP酶比活性降低88%，在浓度为10⁻⁹ M时达到最大效应。这种效应被10⁻⁸ M的N⁶-环己基腺苷（CHA，A₁腺苷受体激动剂）模拟，并被10⁻⁹ M的8-环戊基-1,3-二丙基黄嘌呤（DPCPX，A₁腺苷受体拮抗剂）逆转。另一方面，5'-N-乙基-羧酰胺腺苷（NECA，A₂腺苷受体激动剂）在10⁻⁹ - 10⁻⁵ M范围内使用时，并未改变（Na⁺+K⁺）ATP酶比活性。同样，10⁻⁸ M的3,7-二甲基-1-丙炔基黄嘌呤（DMPX，A₂腺苷受体拮抗剂）也未改变腺苷的抑制作用。这些数据表明，腺苷对长红猎蝽马氏管中（Na⁺+K⁺）ATP酶比活性的抑制作用是由A₁腺苷受体激活介导的。《Archives》
