Purification and characterization of lecithin:cholesterol acyltransferase.
作者信息
Gustow E, Varma K G, Soloff L A
出版信息
Scand J Clin Lab Invest Suppl. 1978;150:1-5. doi: 10.3109/00365517809104891.
PMID:106459
Abstract
The purification of lecithin:cholesterol acyltransferase (LCAT] from human plasma is reported. Hydroxylapatite fractions were approximately 16,000 fold purified over the starting plasma and were free of high-density lipoprotein (HDL) and albumin. The enzyme showed one band on polyacrylamide gel electrophoresis, SDS-urea polyacrylamide gel electrophoresis, isoelectric focusing, and one arc in immunodiffusion against a goat antiserum preparation. It was determined to be a glycoprotein with a molecular weight of approximately 70,000 and a pI of 3.7--4.0.
摘要
相似文献
1
Purification and characterization of lecithin:cholesterol acyltransferase.
Scand J Clin Lab Invest Suppl. 1978;150:1-5. doi: 10.3109/00365517809104891.
2
Isolation and characterization of lecithin-cholesterol acyltransferase from hog plasma.猪血浆中卵磷脂胆固醇酰基转移酶的分离与鉴定。
Biochim Biophys Acta. 1986 Jun 11;877(1):179-90. doi: 10.1016/0005-2760(86)90133-5.
3
Purification of human plasma lecithin:cholesterol acyltransferase by covalent chromatography.
J Biochem Biophys Methods. 1987 Sep;14(6):323-33. doi: 10.1016/0165-022x(87)90026-1.
4
Effect of human plasma apolipoproteins on the activity of purified lecithin: cholesterol acyltransferase.
Artery. 1979 Jan;5(1):61-75.
5
Purification of human plasma lecithin:cholesterol acyltransferase and its activation by metal ions.
Can J Biochem. 1980 Jul;58(7):539-41. doi: 10.1139/o80-073.
6
A method for isolating human plasma lecithin:cholesterol acyltransferase without using anti-apolipoprotein D, and its characterization.
Biochim Biophys Acta. 1983 Jun 16;752(1):89-97. doi: 10.1016/0005-2760(83)90236-9.
7
Isolation, characterization, and assay of lecithin-cholesterol acyltransferase.
Methods Enzymol. 1986;129:763-83. doi: 10.1016/0076-6879(86)29103-x.
8
Purification of horse (Equus caballus) serum lecithin:cholesterol acyltransferase.
Comp Biochem Physiol B. 1987;88(1):363-8. doi: 10.1016/0305-0491(87)90128-3.
9
10
A method for the purification of milligram quantities of stable human phosphatidylcholine-cholesterol acyltransferase.
Biochem J. 1976 Jun 1;155(3):583-8. doi: 10.1042/bj1550583.
Zotero 插件