Tanic N, Dedovic N, Vujosevic M, Dimitrijevic B
Institute for Biological Research Sinisa Stankovic, 11060 Belgrade, Yugoslavia.
Genome Res. 2000 Jan;10(1):55-61.
Using AP-PCR-based DNA profiling we examined some structural features of B chromosomes from yellow-necked mice Apodemus flavicollis. Mice harboring one, two, or three or lacking B chromosomes were examined. Chromosomal structure was scanned for variant bands by using a series of arbitrary primers and from these, informative bands were selected. The selection criteria used were the ability to differentiate between individuals of the species, to detect markers common for both A and B chromosomes, and, importantly, to differentiate between A- and B-chromosome sets. In addition to primers, profiling conditions were found to be critical for meeting the selection criteria. Primers and analysis conditions that demonstrated structural characteristics unique to the B-chromosome set are described. These characteristics included variant bands as qualitative parameters and altered electrophoretic band intensities as quantitative distinctions estimated by integration of densitometric profiles of electrophoretograms. B chromosome-specific molecular markers are easy to detect by AP-PCR-based DNA profiling in the presence of a full set of A chromosomes. Models for the origin of yellow-necked mouse B chromosomes are discussed in the context of presented data.
我们利用基于扩增片段长度多态性聚合酶链反应（AP-PCR）的DNA谱分析技术，研究了黄颈姬鼠（Apodemus flavicollis）B染色体的一些结构特征。我们检测了携带一条、两条或三条B染色体以及不携带B染色体的小鼠。使用一系列任意引物扫描染色体结构以寻找变异条带，并从中选择信息丰富的条带。所采用的选择标准包括能够区分该物种的个体、检测A染色体和B染色体共有的标记，以及重要的是，区分A染色体组和B染色体组。除了引物之外，还发现谱分析条件对于满足选择标准至关重要。本文描述了显示B染色体组独特结构特征的引物和分析条件。这些特征包括作为定性参数的变异条带，以及通过电泳图谱光密度分析谱积分估计的作为定量差异的改变的电泳条带强度。在存在全套A染色体的情况下，通过基于AP-PCR的DNA谱分析很容易检测到B染色体特异性分子标记。本文结合所呈现的数据讨论了黄颈姬鼠B染色体的起源模型。
