克罗地亚克尔克岛人群中HLA II类区域的高分辨率分子分型

High resolution molecular typing of HLA class II region in the population of the island of Krk, Croatia.

作者信息

Grubić Z, Zunec R, Cecuk-Jelicić E, Kastelan D, Kerhin-Brkljacić V, Kastelan A

机构信息

National Referral Organ Transplantation and Tissue Typing Center, University Hospital Rebro, Zagreb, Croatia.

出版信息

Coll Antropol. 1999 Dec;23(2):577-88.

PMID:10646231

Abstract

The HLA class II alleles (DRB1, DRB3, DRB5, DQA1, and DQB1) and haplotypic associations were studied in the population of the island of Krk using the PCR-SSOP method and the 12th International Histocompatibility Workshop primers and probes. Allele and haplotypic frequencies were compared with the general Croatian population. Significant differences were observed between the population of the island of Krk and Croatians for: a) three broad specificities at DRB1 locus (DRB101, 15, and 07), b) one allele at DRB3 locus (DRB30301), c) one allele at DQA1 locus (DQA10201), d) one allele at DQB1 locus (DQB10303). Four unusual haplotypic associations, which have not yet been described in the Croatian population, DRB11301-DQA10103-DQB10607, DRB11302-DQA10102-DQB10605, DRB11305-DQA10102-DQB10605 and DRB11305-DQA10103-DQB10603 were observed in the population from the island of Krk.

摘要

采用PCR - SSOP方法以及第12届国际组织相容性研讨会的引物和探针，对克尔克岛人群中的HLA - II类等位基因（DRB1、DRB3、DRB5、DQA1和DQB1）及单倍型关联进行了研究。将等位基因和单倍型频率与克罗地亚普通人群进行了比较。结果发现，克尔克岛人群与克罗地亚人群在以下方面存在显著差异：a）DRB1位点的三种广泛特异性（DRB101、15和07）；b）DRB3位点的一个等位基因（DRB30301）；c）DQA1位点的一个等位基因（DQA10201）；d）DQB1位点的一个等位基因（DQB10303）。在克尔克岛人群中观察到四种尚未在克罗地亚人群中描述过的异常单倍型关联，即DRB11301 - DQA10103 - DQB10607、DRB11302 - DQA10102 - DQB10605、DRB11305 - DQA10102 - DQB10605和DRB11305 - DQA10103 - DQB10603。