Hanocq-Quertier J, Baltus E, Brachet J
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2028-32. doi: 10.1073/pnas.73.6.2028.
Treatment of small-(stage III) or medium-sized (stage IV) Xenopus laevis oocytes with progesterone, human chorionic gonadotropin, or para-hydroxymercuriphenylsulfonate does not induce maturation. Only the full-grown oocytes (stage VI) undergo maturation when treated with either one of these three substances. In contrast, injection of maturation promoting factor into oocytes of stages III-VI invariably leads to chromosome condensation and germinal vesicle breakdown. No maturation spindle is found in oocytes smaller than 0.9 nm in diameter, and the nuclear sap does not mix with the cytoplasm in the smallest (0.45-0.55 mm in diameter) oocytes. In oocytes of 0.9 mm, maturation is identical to that of full-grown oocytes, except that the maturation spindle does not reach the cortex of the oocyte. Progesterone increases protein synthesis in medium-sized (0.8 mm in diameter) oocytes without inducing meiosis. It has little or no effect on protein synthesis in smaller oocytes.
用孕酮、人绒毛膜促性腺激素或对羟基汞苯磺酸盐处理小型（III期）或中型（IV期）非洲爪蟾卵母细胞不会诱导其成熟。只有完全成熟的卵母细胞（VI期）在用这三种物质中的任何一种处理时才会发生成熟。相反，将成熟促进因子注入III - VI期的卵母细胞中总是会导致染色体凝聚和生发泡破裂。在直径小于0.9纳米的卵母细胞中未发现成熟纺锤体，并且在最小的（直径0.45 - 0.55毫米）卵母细胞中核液不与细胞质混合。在直径为0.9毫米的卵母细胞中，成熟过程与完全成熟的卵母细胞相同，只是成熟纺锤体未到达卵母细胞的皮质。孕酮可增加中型（直径0.8毫米）卵母细胞中的蛋白质合成，但不诱导减数分裂。它对较小卵母细胞中的蛋白质合成几乎没有影响。
