Zhu Q, Zeng C, Huhalov A, Yao J, Turi T G, Danley D, Hynes T, Cong Y, DiMattia D, Kennedy S, Daumy G, Schaeffer E, Marasco W A, Huston J S
IntraImmune Therapies Inc., P.O. Box 15599, Boston, MA 02215-0011,
J Immunol Methods. 1999 Dec 10;231(1-2):207-22. doi: 10.1016/s0022-1759(99)00158-1.
8 h) and high steady-state levels of protein accumulation, while the H2 intrabodies had a half-life of 2 h and less protein at steady state. These results suggest that the choice of sFv as an intrabody depends critically on the intracellular sFv protein having an extended half-life and elevated steady-state level. Thus, extended half-life must be considered together with sFv antibody specificity and affinity when choosing an optimal sFv intrabody for functional studies of cellular proteins.
8小时）以及较高的蛋白质积累稳态水平，而H2细胞内抗体的半衰期为2小时且稳态时蛋白质含量较低。这些结果表明，选择单链抗体片段作为细胞内抗体关键取决于细胞内单链抗体片段蛋白具有延长的半衰期和升高的稳态水平。因此，在为细胞蛋白功能研究选择最佳单链抗体片段细胞内抗体时，必须将延长的半衰期与单链抗体片段的特异性和亲和力一起考虑。
