Matek M, Blanusa M, Grgić J
Institute for Medical Research and Occupational Health, Zagreb, Croatia.
Arh Hig Rada Toksikol. 1999 Sep;50(3):283-8.
This study describes and compares two methods of atomic absorption spectrometry (AAS) for the selenium (Se) analysis in food: electrothermal AAS (ET AAS) and hydride generation method of AAS (HG AAS). The accuracy of the two methods was established by analysing two biological reference materials: Wheat Flour 1567a and Bovine Liver 1577b from the National Institute of Standards and Technology, USA. Good agreement with certified values was obtained for both methods. The accuracy of ET AAS method is 109% and 103% for Wheat Flour and Bovine Liver, respectively. The respective accuracies for HG AAS method were 88% and 87%. The detection limit obtained for ET AAS was 1 microgram Se/L and for HG AAS 0.02 microgram Se/L. The repeatability of ET AAS method was 5-11% and that of HG AAS 14-17%. Both methods are similar in accuracy and repeatability, but hydride generation (HG AAS) is more sensitive than graphite furnace technique (ET AAS) for determination of selenium in food.
本研究描述并比较了食品中硒（Se）分析的两种原子吸收光谱法（AAS）：电热原子吸收光谱法（ET AAS）和氢化物发生原子吸收光谱法（HG AAS）。通过分析两种生物参考物质来确定这两种方法的准确性：美国国家标准与技术研究院的小麦粉1567a和牛肝1577b。两种方法均与认证值取得了良好的一致性。ET AAS法对小麦粉和牛肝的准确度分别为109%和103%。HG AAS法的相应准确度分别为88%和87%。ET AAS法的检出限为1微克Se/L，HG AAS法的检出限为0.02微克Se/L。ET AAS法的重复性为5 - 11%，HG AAS法的重复性为14 - 17%。两种方法在准确度和重复性方面相似，但在食品中硒的测定方面，氢化物发生法（HG AAS）比石墨炉技术（ET AAS）更灵敏。
