Chou D K, Tobet S, Jungalwala F B
Department of Biomedical Sciences, Eunice Kennedy Shriver Center for Mental Retardation, Waltham, Massachusetts, USA.
J Neurosci Res. 2000 Jan 15;59(2):188-201. doi: 10.1002/(sici)1097-4547(20000115)59:2<188::aid-jnr5>3.0.co;2-m.
Sulfoglucuronyl carbohydrate (SGC) is expressed on several neural cell-adhesion molecules and on glycolipids. SGC and its binding protein, SBP-1 are developmentally regulated in the nervous system and have been implicated in regulating neurite outgrowth and cell-cell recognition during neuronal cell migration. To elucidate the role of interaction between SGC and SBP-1, microexplant cultures of postnatal day 5 rat cerebellum were employed. In explant cultures, SGC was localized primarily in the neuronal cell processes, neurofilaments, and dendrites that emerge from the core of the explants up to 90 microm, after 24 hr in culture. SGC was also present in the short astrocytic processes near the core of the explant. SBP-1 was localized mainly in the granule neuron cell bodies and faintly on cell plasma membranes and processes. Granule neurons, expressing SBP-1, migrated outward in close contact with the SGC bearing neuronal processes, suggesting interaction between SGC and SBP-1. The neurite outgrowth and cell migration were specifically and severely reduced, in dose-dependent manners, by anti-SGC (HNK-1) and anti-SBP-1 antibodies and sulfoglucuronyl glycolipid (SGGL). Other irrelevant antibodies and glycolipids had little effect. The results showed that SBP-1 was required for neurite outgrowth and that SGC-SBP-1 interaction was important for cell-cell recognition and cell migration.
硫葡糖醛酸碳水化合物（SGC）在多种神经细胞黏附分子和糖脂上表达。SGC及其结合蛋白SBP - 1在神经系统中受到发育调控，并与神经元细胞迁移过程中神经突生长和细胞间识别的调节有关。为了阐明SGC与SBP - 1之间相互作用的作用，采用了出生后第5天大鼠小脑的微外植体培养。在外植体培养中，培养24小时后，SGC主要定位于从外植体核心伸出长达90微米的神经元细胞突起、神经丝和树突中。SGC也存在于外植体核心附近的短星形细胞突起中。SBP - 1主要定位于颗粒神经元细胞体，在细胞质膜和突起上微弱表达。表达SBP - 1的颗粒神经元与带有SGC的神经元突起紧密接触向外迁移，表明SGC与SBP - 1之间存在相互作用。抗SGC（HNK - 1）、抗SBP - 1抗体和硫葡糖醛酸糖脂（SGGL）以剂量依赖的方式特异性且严重地减少了神经突生长和细胞迁移。其他无关抗体和糖脂影响很小。结果表明，神经突生长需要SBP - 1，并且SGC - SBP - 1相互作用对于细胞间识别和细胞迁移很重要。
