Tsuji N, Ohta M, Kawazu S, Kamio T, Isobe T, Shimura K, Fujisaki K
National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
J Vet Med Sci. 1999 Dec;61(12):1331-3. doi: 10.1292/jvms.61.1331.
DNA polymorphism in twelve starains of Eimeria tenella isolated from various places in Japan was examined using 1.l kb small subunits ribosomal RNA amplified by PCR. Genetic variation was evaluated by random amplification of polymorphic DNA (RAPD) analysis. DNA fingerprint patterns were grouped into two, indicating that at least two DNA polymorphisms exist in Japanese E. tenella strains.
利用聚合酶链反应（PCR）扩增的1.1 kb小亚基核糖体RNA，检测了从日本各地分离出的12株柔嫩艾美耳球虫的DNA多态性。通过随机扩增多态性DNA（RAPD）分析评估遗传变异。DNA指纹图谱分为两类，表明日本柔嫩艾美耳球虫菌株中至少存在两种DNA多态性。
