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An indexed genomic library for Paramecium complementation cloning.

作者信息

Keller A M, Cohen J

机构信息

Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.

出版信息

J Eukaryot Microbiol. 2000 Jan-Feb;47(1):1-6. doi: 10.1111/j.1550-7408.2000.tb00001.x.

DOI:10.1111/j.1550-7408.2000.tb00001.x
PMID:10651287
Abstract

Recent pioneering work opened the way for cloning genes in Paramecium by functional complementation of mutants. We present here the construction and pilot utilization of a new indexed library of Paramecium macronuclear DNA. The library is made of 61,440 clones containing inserts mostly between 6 and 12 kilobases. It has already allowed the complementation cloning of four new genes, and this library has proven to be very useful for rapid hybridization cloning.

摘要

相似文献

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