Yang M, Chishima T, Wang X, Baranov E, Shimada H, Moossa A R, Hoffman R M
AntiCancer, Inc., San Diego, CA 92111, USA.
Clin Exp Metastasis. 1999 Jul;17(5):417-22. doi: 10.1023/a:1006665112147.
Stable high-level green fluorescent protein (GFP)-expressing Chinese hamster ovary cells (CHO) were used to visualize the degree of metastatic behavior of this cell line in nude and SCID mice. A stable GFP high-expression CHO clone, selected in 1.5 microM methotrexate, was injected subcutaneously in nude and severe combined immunodeficient (SCID) mice and implanted orthotopically in the ovary of nude mice. CHO proved to be highly metastatic from both the subcutaneous and orthotopic sites as brightly visualized by GFP fluorescence. High-level GFP-expression allowed the visualization of metastatic tumor in fresh live host tissue in great detail. Metastases were visualized by GFP expression in the lung, pleural membrane, spleen, kidney, ovary, adrenal gland, and peritoneum after orthotopic implantation in nude mice. Metastases were visualized by GFP expression mainly in the lung, pleural membrane after subcutaneous implantation in nude mice. Metastases were visualized in the lung and pleural membrane, liver, kidney, and ovary after subcutaneous implantation in SCID mice. The construction of highly fluorescent stable GFP transfectants of CHO has revealed the multi-organ metastatic capability of CHO cells. CHO has such a high degree of malignancy that it is metastatic from both the orthotopic and subcutaneous transplant sites. This highly malignant GFP-expressing cell-line with multi-organ metastatic affinity should serve as a powerful tool to study tumor-host interaction.
利用稳定表达高水平绿色荧光蛋白（GFP）的中国仓鼠卵巢细胞（CHO）来观察该细胞系在裸鼠和严重联合免疫缺陷（SCID）小鼠体内的转移行为程度。在1.5微摩尔甲氨蝶呤中筛选出的稳定高表达GFP的CHO克隆，皮下注射到裸鼠和严重联合免疫缺陷（SCID）小鼠体内，并原位植入裸鼠卵巢。通过GFP荧光清晰可见，CHO从皮下和原位部位均具有高度转移性。高水平的GFP表达使得能够非常详细地观察新鲜活体宿主组织中的转移性肿瘤。在裸鼠原位植入后，通过GFP表达在肺、胸膜、脾脏、肾脏、卵巢、肾上腺和腹膜中观察到转移。在裸鼠皮下植入后，通过GFP表达主要在肺、胸膜中观察到转移。在SCID小鼠皮下植入后，在肺、胸膜、肝脏、肾脏和卵巢中观察到转移。CHO高荧光稳定GFP转染子的构建揭示了CHO细胞的多器官转移能力。CHO具有如此高的恶性程度，以至于它从原位和皮下移植部位都能发生转移。这种具有多器官转移亲和力的高恶性GFP表达细胞系应成为研究肿瘤-宿主相互作用的有力工具。
