Forsythe P, McGarvey L P, Heaney L G, MacMahon J, Ennis M
Department of Clinical Biochemistry, The Queen's University of Belfast,UK.
Clin Exp Allergy. 2000 Feb;30(2):225-32. doi: 10.1046/j.1365-2222.2000.00770.x.
Sensory neuropeptides have been suggested to play a role in the pathogenesis of a number of respiratory diseases including asthma and chronic non-productive cough.
To investigate the action of sensory neuropeptides on airway mast cells obtained by bronchoalveolar lavage (BAL).
BAL was performed on 23 nonasthmatic patients with cough (NAC), 11 patients with cough variant asthma (CVA) and 10 nonatopic controls. Washed lavage cells were stimulated (20 min, 37 degrees C) with calcitonin gene-related peptide (CGRP), neurokinin A (NKA) and substance P (25 and 50 micromol/L).
The neuropeptides tested induced histamine release in all groups studied. Only CGRP (50 micromol/L) induced significantly more histamine release from both NAC and CVA patients compared with control subjects (P = 0.038 and 0.045, respectively).
Regardless of aetiology, mast cells from patients with chronic cough appear to have an increased responsiveness to CGRP compared with controls. The results of the present study suggest that the role of CGRP in chronic cough should be further investigated.
感觉神经肽被认为在包括哮喘和慢性干咳在内的多种呼吸系统疾病的发病机制中起作用。
研究感觉神经肽对通过支气管肺泡灌洗（BAL）获得的气道肥大细胞的作用。
对23例非哮喘性咳嗽患者（NAC）、11例咳嗽变异性哮喘患者（CVA）和10例非特应性对照者进行支气管肺泡灌洗。用降钙素基因相关肽（CGRP）、神经激肽A（NKA）和P物质（25和50 μmol/L）刺激洗涤后的灌洗细胞（20分钟，37摄氏度）。
所测试的神经肽在所有研究组中均诱导组胺释放。与对照组相比，仅CGRP（50 μmol/L）诱导NAC和CVA患者释放的组胺明显更多（分别为P = 0.038和0.045）。
无论病因如何，与对照组相比，慢性咳嗽患者的肥大细胞对CGRP的反应性似乎增强。本研究结果表明，应进一步研究CGRP在慢性咳嗽中的作用。
