Matzk F, Meister A, Schubert I
Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrabetae 3, D-06466 Gatersleben, Germany.
Plant J. 2000 Jan;21(1):97-108. doi: 10.1046/j.1365-313x.2000.00647.x.
Seed samples of 32 species (obligate and facultative sexuals and apomicts of monocots and dicots) were investigated by flow cytometry to reveal the pathway of reproduction. Ten different pathways of seed formation could be reconstructed considering whether the female and/or male gametes were reduced or unreduced, the embryos arose via the zygotic or parthenogenetic route and the endosperm via the pseudogamous or autonomous route. The screen is suited to select sporophytic or gametophytic mutants in sexual species, to identify pure sexual or obligate apomictic genotypes from facultative apomictic species, and to analyze the inheritance of the individual reproductive processes. Corresponding unique results are presented for Arabidopsis, Arabis, Hypericum and Poa. The screen of mature seeds by flow cytometry yielded more information about the reproductive behavior of individual plants than any other available test, and is very useful both in basic research and plant breeding.
通过流式细胞术对32个物种（单子叶植物和双子叶植物的专性有性生殖、兼性有性生殖和无融合生殖类型）的种子样本进行了研究，以揭示其繁殖途径。考虑到雌配子和/或雄配子是减数的还是未减数的、胚是通过合子途径还是孤雌生殖途径产生的以及胚乳是通过假受精途径还是自主途径产生的，可重建出10种不同的种子形成途径。该筛选方法适用于在有性生殖物种中选择孢子体或配子体突变体，从兼性无融合生殖物种中鉴定纯有性生殖或专性无融合生殖基因型，以及分析各个生殖过程的遗传情况。文中给出了拟南芥、南芥、金丝桃属植物和早熟禾属植物相应的独特结果。通过流式细胞术对成熟种子进行筛选，比任何其他现有测试都能获得更多关于单株植物生殖行为的信息，在基础研究和植物育种中都非常有用。
