Söderlund J, Nilsson C, Ekman M, Walther L, Gaines H, Biberfeld G, Biberfeld P
Immunopathology Laboratory, Karolinska Institutet, Stockholm, Sweden.
Scand J Immunol. 2000 Feb;51(2):186-94. doi: 10.1046/j.1365-3083.2000.00679.x.
This study shows that characteristic dendritic, antigen presenting cells, can be generated from adherent peripheral blood mononuclear cells (PBMC)/monocytes of uninfected and SIVsm-infected cynomolgus monkeys after stimulation in vitro with granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin (IL)-4. The recruitment of monocyte derived dendritic cells (MDDC) was usually possible irrespective of the level of immunodeficiency (CD4-level) and viremia. The cynomolgus MDDC closely resembled their human counterpart (immature MDDC) with regard to capacity to upregulate CD1a, CD40, CD86 and human leukocyte antigen (HLA)-DR and develop dendrites and veiled processes. Such MDDC also increased their capacity for antigen uptake (dextran endocytoses/macropinocytosis) and for induction of T-cell proliferation in mixed leukocyte reaction (MLR) assays. However, although no clear difference with regard to phenotype and morphology was seen between MDDC from SIV-infected and uninfected monkeys, a reduction in MLR responsiveness in MDDC from SIV infected monkeys was consistently detected within each experiment.
本研究表明，在用粒细胞巨噬细胞集落刺激因子（GM-CSF）和白细胞介素（IL）-4体外刺激后，未感染和感染SIVsm的食蟹猴的贴壁外周血单个核细胞（PBMC）/单核细胞可生成具有特征性树突状的抗原呈递细胞。无论免疫缺陷水平（CD4水平）和病毒血症如何，通常都有可能招募单核细胞衍生的树突状细胞（MDDC）。食蟹猴MDDC在上调CD1a、CD40、CD86和人类白细胞抗原（HLA）-DR以及形成树突和面纱样突起的能力方面与人类对应细胞（未成熟MDDC）极为相似。此类MDDC在混合淋巴细胞反应（MLR）试验中还增强了其抗原摄取能力（葡聚糖内吞作用/巨吞饮作用）和诱导T细胞增殖的能力。然而，尽管在感染SIV和未感染猴子的MDDC之间未观察到明显的表型和形态差异，但在每个实验中均始终检测到感染SIV猴子的MDDC的MLR反应性降低。
