Oskam N T, Bijleveld E H, Hulsebos T J
Institute of Human Genetics, Academic Medical Center, University of Amsterdam, The Netherlands.
Int J Cancer. 2000 Feb 1;85(3):336-9.
Loss of heterozygosity for chromosome 22 (LOH 22) occurs in gliomas of all malignancy grades. Neurofibromatosis type 2 (NF2) patients are at increased risk of developing a glioma. However, the NF2 gene in 22q12.2 is not involved in glioma tumorigenesis. To detect additional regions on chromosome 22 that may harbor tumor suppressor genes important in glioma tumorigenesis, we determined LOH 22 profiles for 159 gliomas using 32 markers. LOH 22 was found in 46 tumors (29%). Thirteen tumors displayed partial LOH 22, from which we deduced a region of common deletion between markers D22S928 and D22S1169 in 22q13.3. LOH of at least this region was detected in 13% of the astrocytomas (As), in 20% of the anaplastic astrocytomas (AAs) and in 35% of the glioblastomas multiforme (GBMs). The significant increased frequency of LOH 22q13.3 in the highest malignancy grade (GBM vs. A and AA, p = 0.02) indicates that loss of this region is associated with astrocytoma progression.
22号染色体杂合性缺失（LOH 22）在所有恶性程度等级的胶质瘤中均有发生。2型神经纤维瘤病（NF2）患者发生胶质瘤的风险增加。然而，位于22q12.2的NF2基因并不参与胶质瘤的肿瘤发生过程。为了检测22号染色体上可能含有对胶质瘤肿瘤发生至关重要的肿瘤抑制基因的其他区域，我们使用32个标记物确定了159例胶质瘤的LOH 22图谱。在46个肿瘤（29%）中发现了LOH 22。13个肿瘤显示部分LOH 22，由此我们推断出22q13.3区域中标记物D22S928和D22S1169之间存在一个共同缺失区域。在13%的星形细胞瘤（As）、20%的间变性星形细胞瘤（AAs）和35%的多形性胶质母细胞瘤（GBMs）中检测到至少该区域的LOH。在最高恶性程度等级中（GBM与A和AA相比，p = 0.02），22q13.3区域LOH频率显著增加，这表明该区域的缺失与星形细胞瘤进展相关。
