Griffin T J, Smith L M
Department of Molecular Biotechnology, University of Washington, Box 357730, Seattle, WA 98195-7730, USA.
Trends Biotechnol. 2000 Feb;18(2):77-84. doi: 10.1016/s0167-7799(99)01401-8.
Single-nucleotide polymorphisms (SNPs) have great potential for use in genetic-mapping studies, which locate and characterize genes that are important in human disease and biological function. For SNPs to realize their full potential in genetic analysis, thousands of different SNP loci must be screened in a rapid, accurate and cost-effective manner. Matrix-assisted laser desorption-ionization-time-of-flight (MALDI-TOF) mass spectrometry is a promising tool for the high-throughput screening of SNPs, with future prospects for use in genetic analysis.
单核苷酸多态性（SNP）在基因定位研究中具有巨大潜力，该研究可定位并表征在人类疾病和生物学功能中起重要作用的基因。为了使SNP在基因分析中充分发挥其潜力，必须以快速、准确且经济高效的方式筛选数千个不同的SNP位点。基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱是一种用于SNP高通量筛选的有前景的工具，在基因分析中具有广阔的应用前景。
