Doboszynska T, Andronowska A, Janiszewska L, Sobotka A
Department of Reproductive Histophysiology, Polish Academy of Sciences, Olsztyn, Poland.
Lymphology. 1999 Dec;32(4):132-50.
The broad ligament containing uterine, paraovarian, and oviduct lymphatics was examined in the pig in various phases of the estrous cycle using light, scanning and transmission electron microscopy. The architecture of these regions differed and was independent of the lymphangions of the precollector and collector lymphatic vessels. Lymphangions were separated from mesothelium by connective tissue and/or muscle layers; however, in the vicinity of the thin walled paraovarian sac, large lymphangions were often compressed between two epithelial layers. Numerous lymphatic lacunae were in direct contact with the peritoneal and paraovarian sac cavities. The mesothelial lining of the broad ligament and the external and internal epithelium of the pig paraovarian sac displayed two distinct cell types. Only smaller cuboidal cells with prominent microvilli extended above the lymphatic endothelium. The surfaces of these cells were discontinuous and showed: 1) lymphatic stomata, 2) small pores or fenestrae, 3) a superficial network of epithelial-free communications with underlying connective tissue to the paraovarian sac in the postovulatory period independent of the lymphatic vasculature, and 4) endothelial (instead of epithelial) cells with crevice-like discontinuities in large portions of the internal sac surface during the follicular phase of estrus. Numerous lymphatic stomata had orifices composed of flattened cuboidal cells while lymphatic endothelial cells were characterized by macula or zonula adherent connections formed within rims of various sizes (up to 50 microns in diameter). During estrus, there were circular (0.5-2.0 microns) and irregular (to 10 microns) interendothelial openings in stomatal orifices with migrating cells. These morphologic findings suggest that absorption and passage of fluid, particles and cells between cavities and the lymphatic lumen in areas of the paraovarian lymphatic plexus in the pig is feasible.
利用光学显微镜、扫描电子显微镜和透射电子显微镜，对处于发情周期不同阶段的猪的阔韧带（包含子宫、卵巢旁和输卵管淋巴管）进行了检查。这些区域的结构有所不同，且与收集前和收集淋巴管的淋巴管腔无关。淋巴管腔通过结缔组织和/或肌肉层与间皮分离；然而，在薄壁的卵巢旁囊附近，大的淋巴管腔常被挤压在两层上皮之间。许多淋巴腔隙与腹膜和卵巢旁囊腔直接接触。阔韧带的间皮衬里以及猪卵巢旁囊的外上皮和内上皮显示出两种不同的细胞类型。只有带有突出微绒毛的较小立方形细胞延伸至淋巴管内皮上方。这些细胞的表面不连续，表现为：1）淋巴小孔，2）小孔或窗孔，3）在排卵后期与卵巢旁囊下方结缔组织的无上皮连通的浅表网络，与淋巴脉管系统无关，以及4）在发情卵泡期，囊内表面大部分区域具有缝隙状不连续的内皮（而非上皮）细胞。许多淋巴小孔具有由扁平立方形细胞组成的孔口，而淋巴管内皮细胞的特征是在各种大小（直径可达50微米）的边缘内形成的粘着斑或带状连接。在发情期，气孔孔口中存在圆形（0.5 - 2.0微米）和不规则（达10微米）的内皮间开口，并有迁移细胞。这些形态学发现表明，猪卵巢旁淋巴丛区域的腔隙与淋巴腔之间的液体、颗粒和细胞的吸收及通过是可行的。
