Toro F, Lopera F, Ossa J, Madrigal L, Mira A, Díaz A, Parra S
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.
Rev Neurol. 1999;29(12):1104-7.
To investigate some immune component that could contribute to the pathogenesis of Alzheimer's disease associated with the E280A mutation of the presenilin-1 gene (PS-1).
Serum antibodies against both cardiolipin and beta-amyloid peptides (beta 1-40 and beta 1-42) were quantitated by means of an indirect ELISA technique in carriers of the mutation, either healthy or with Alzheimer's disease, as well as in normal controls without the mutation.
Out of 19 patients with Alzheimer's disease only 2 had increased values of antibodies to cardiolipin, namely: one with 14.58 micrograms/ml of IgM and the other with 36.16 micrograms/ml of IgG. The remaining individuals revealed values under 10 micrograms/ml, considered normal, and there was no significant difference between the groups. Significant serum reactivity (p < 0.001) was detected against both beta-amyloid peptides in the mutation carriers, either with or without Alzheimer's disease, as compared to the control group. No correlation was detected between this antibody response and the mental or functional situation of the patients.
beta-amyloid antibodies, present in the mutation carriers, may simply represent a marker of immune activation induced by beta-amyloid with no in vivo effect; however, despite the results, the possibility can not be ruled out of a pathogenic role of these antibodies in early onset Alzheimer's disease.
研究某些可能与早老素 -1基因（PS -1）E280A突变相关的阿尔茨海默病发病机制有关的免疫成分。
采用间接ELISA技术，对携带该突变的健康者或患有阿尔茨海默病者以及无该突变的正常对照者血清中的抗心磷脂抗体和β-淀粉样肽（β1-40和β1-42）抗体进行定量分析。
在19例阿尔茨海默病患者中，只有2例抗心磷脂抗体值升高，即：1例IgM为14.58微克/毫升，另1例IgG为36.16微克/毫升。其余个体的值均低于10微克/毫升，属于正常范围，且各组之间无显著差异。与对照组相比，在携带突变的患者中，无论是否患有阿尔茨海默病，均检测到针对两种β-淀粉样肽的显著血清反应性（p < 0.001）。未检测到这种抗体反应与患者的精神或功能状况之间存在相关性。
突变携带者体内存在的β-淀粉样肽抗体可能仅仅是β-淀粉样肽诱导的免疫激活的标志物，在体内无作用；然而，尽管有这些结果，仍不能排除这些抗体在早发型阿尔茨海默病中具有致病作用的可能性。
