Hutton W C, Garbow J R, Hayes T R
Nutrition and Consumer Sector, Monsanto, St. Louis, Missouri 63167, USA.
Lipids. 1999 Dec;34(12):1339-46. doi: 10.1007/s11745-999-0487-0.
Magic-angle spinning (MAS) 13C nuclear magnetic resonance (NMR) spectroscopy is a convenient method for nondestructive, quantitative characterization of seed oil composition. We describe results for intact hybrid and transformed canola seeds. The MAS 13C NMR technique complements and agrees with gas chromatography results. The spectral resolution approaches that of neat, liquid oils. MAS 13C NMR data allow quantitative analysis of major oil components, including saturates and oleic, linoleic, and linolenic acyl chains. 13C NMR directly and quantitatively elucidates, triglyceride regiochemistry and acyl chain cis-trans isomers that cannot be quickly detected by other methods. MAS 13C NMR can serve as the primary method for development of near-infrared seed oil calibrations. These NMR methods are nondestructive and attractive for plant-breeding programs or other studies (e.g., functional genomics) where loss of seed viability is inconvenient.
魔角旋转（MAS）13C核磁共振（NMR）光谱法是一种用于种子油成分无损定量表征的便捷方法。我们描述了完整杂交和转基因油菜籽的相关结果。MAS 13C NMR技术与气相色谱结果互补且一致。光谱分辨率接近纯液态油的分辨率。MAS 13C NMR数据允许对主要油成分进行定量分析，包括饱和脂肪酸以及油酸、亚油酸和亚麻酸酰基链。13C NMR直接且定量地阐明了甘油三酯区域化学和酰基链顺反异构体，而这些是其他方法无法快速检测到的。MAS 13C NMR可作为近红外种子油校准开发的主要方法。这些NMR方法具有无损性，对于植物育种计划或其他种子活力丧失不便的研究（如功能基因组学）具有吸引力。
