Park Y I, Bonetti S J, Gander J E
Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611-0700, USA.
Appl Environ Microbiol. 2000 Feb;66(2):832-5. doi: 10.1128/AEM.66.2.832-835.2000.
Addition of 3 M NaCl to 72-h cultures of Penicillium fellutanum in 2 mM phosphate resulted in an increase in percentage of extracellular peptidophosphogalactomannan III (pP(x)GM(iii)) and a decrease in that of pP(x)GM(ii). The magnitude of (31)P nuclear magnetic resonance signals at 1.47 and 1.33 ppm of phospho-1-O-[N-peptidyl-(2-aminoethanol)] phosphodiesters pP(x)GM(ii) and pP(x)GM(iii) decreased compared with controls. The data suggest that serine, glycine, and threonine residues from the 3-kDa peptide and from galactofuranosyl-6-O-phospho-1'-O-[N-peptidyl-(2-aminoethanol)] residues were the precursors of the needed choline-derived osmolytes.
向在2 mM磷酸盐中培养72小时的费鲁塔青霉培养物中添加3 M氯化钠,导致细胞外肽磷酸半乳甘露聚糖III(pP(x)GM(iii))的百分比增加,而pP(x)GM(ii)的百分比降低。与对照相比,pP(x)GM(ii)和pP(x)GM(iii)的磷酸-1-O-[N-肽基-(2-氨基乙醇)]磷酸二酯在1.47和1.33 ppm处的³¹P核磁共振信号强度降低。数据表明,来自3 kDa肽以及半乳呋喃糖基-6-O-磷酸-1'-O-[N-肽基-(2-氨基乙醇)]残基的丝氨酸、甘氨酸和苏氨酸残基是所需胆碱衍生渗透剂的前体。