[CDKN2/p16基因甲基化失活与肺癌的相关性]
[Correlation of inactivation of CDKN2/p16 gene by methylation and lung cancers].
作者信息
Su C, Shan X
机构信息
Department of Molecular Biology, Nanjing 81 Hospital, Nanjing, Jiangsu, 210002 P.R. China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2000 Feb;17(1):36-8.
OBJECTIVE
To study the relationship between methylation state of CDKN2/p16 gene and lung cancers.
METHODS
Genomic DNA was digested with methylation-sensitive enzymes and Southern blotting was used to analyses the methylation state of CDKN2/p16 gene in 89 cases of lung cancer.
RESULTS
Twenty-one out of 89 lung cancers(23.6%) shown methylation of CDKN2/p16 gene, and 17 of them occurred in 42 P16 negative cases with a rate of 40.5%(17/42).
CONCLUSION
Methylation of CDKN2/p16 gene 5' CpG island is probably an important mechanism of gene inactivation; it may take part in the onset and process of lung cancer.
目的
研究CDKN2/p16基因甲基化状态与肺癌的关系。
方法
用甲基化敏感酶消化基因组DNA，采用Southern印迹法分析89例肺癌中CDKN2/p16基因的甲基化状态。
结果
89例肺癌中有21例（23.6%）显示CDKN2/p16基因甲基化，其中17例发生在42例P16阴性病例中，发生率为40.5%（17/42）。
结论
CDKN2/p16基因5' CpG岛甲基化可能是基因失活的重要机制；它可能参与肺癌的发生和发展过程。
Zotero 插件