[急性髓系白血病中胰岛素样生长因子II基因印记缺失]
[Loss of imprinting of insulin-like growth factor II gene in acute myeloid leukemia].
作者信息
Liu Y, Yang B, Zhu H, Wang J
机构信息
Institute of Clinical Medical Sciences, Affiliated Hospital, Wannan Medical College, Wuhu, Anhui, 241001 P.R. China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2000 Feb;17(1):39-41.
OBJECTIVE
To investigate the status of insulin- like growth factor-2(IGF-2) gene imprinting in acute myeloid leukemia(AML).
METHODS
Selection of heterozygous cases for Apa I polymorphism within exon 9 of IGF-2 gene and further analysis of loss of imprinting(LOI) were done in 26 AML patients by polymerase chain reaction(PCR), reverse transcription PCR (RT-PCR) and restriction fragment length polymorphism(RFLP) technique.
RESULTS
Eleven cases of AML patients were identified as heterozygous for IGF-2(11/26); 8 heterozygous patients showed biallelic expression of IGF-2, indicating loss of IGF-2 imprinting.
CONCLUSION
The frequent LOI of IGF-2 may play a role in the development of acute myeloid leukemia.
目的
探讨胰岛素样生长因子2（IGF-2）基因印记在急性髓系白血病（AML）中的状态。
方法
选取26例AML患者，采用聚合酶链反应（PCR）、逆转录PCR（RT-PCR）及限制性片段长度多态性（RFLP）技术，对IGF-2基因第9外显子Apa I多态性的杂合子病例进行筛选，并进一步分析印记缺失（LOI）情况。
结果
26例AML患者中有11例被鉴定为IGF-2杂合子（11/26）；8例杂合子患者显示IGF-2双等位基因表达，提示IGF-2印记缺失。
结论
IGF-2频繁的印记缺失可能在急性髓系白血病的发生发展中起作用。
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