Duzgun S A, Rasque H, Kito H, Azuma N, Li W, Basson M D, Gahtan V, Dudrick S J, Sumpio B E
Department of Surgery, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
J Cell Biochem. 2000 Jan;76(4):567-71. doi: 10.1002/(sici)1097-4644(20000315)76:4<567::aid-jcb5>3.0.co;2-w.
The effect of hyperosmolarity on the induction of the mitogen-activated protein kinases (MAPK) was studied in bovine aortic endothelial cell (EC). Different types of agents were used to differentiate the effects of osmolarity from other variables. Hypertonic treatment with physiologically relevant levels of NaCl (350 mOsm/kg H(2)O) significantly increased the level of expression of p38 within 2 min, and ERK-1/2 and JNK after 10 min. The inductions peaked between 30 and 60 min and returned to baseline levels within 2 h. A similar pattern of induction occurred with ionic contrast agent. p38 induction by glucose and mannitol showed a similar pattern, although the level of ERK-1/2 phosphorylation was not as robust, and JNK was not induced by glucose. Urea did not affect the level of induction of the MAPK isoforms. It is concluded that MAPK plays an important role in hyperosmolality-induced signal transduction. Different osmotic agents induce MAPK expression differently. No MAPK induction with urea implies that cell shrinkage may be an important component of hyperosmolality-induced MAPK phosphorylation.
在牛主动脉内皮细胞（EC）中研究了高渗对丝裂原活化蛋白激酶（MAPK）诱导的影响。使用不同类型的试剂来区分渗透压与其他变量的影响。用生理相关水平的NaCl（350 mOsm/kg H₂O）进行高渗处理，在2分钟内显著增加了p38的表达水平，10分钟后增加了ERK-1/2和JNK的表达水平。诱导作用在30至60分钟达到峰值，并在2小时内恢复到基线水平。离子型造影剂也出现了类似的诱导模式。葡萄糖和甘露醇对p38的诱导显示出类似的模式，尽管ERK-1/2磷酸化水平不那么强烈，且葡萄糖未诱导JNK。尿素不影响MAPK亚型的诱导水平。结论是，MAPK在高渗诱导的信号转导中起重要作用。不同的渗透剂对MAPK表达的诱导方式不同。尿素不诱导MAPK表明细胞收缩可能是高渗诱导MAPK磷酸化的重要组成部分。
