Peters S, Kayatz P, Kociok N, Heimann K, Schraermeyer U
Department of Vitreoretinal Surgery, University Eye Clinic, Cologne, Germany.
Graefes Arch Clin Exp Ophthalmol. 1999 Dec;237(12):976-83. doi: 10.1007/s004170050333.
The fate of indigestible material injected into the subretinal space of rats was investigated.
The non-toxic dye Monastral Blue (MB), which cannot be digested within the lysosomal compartment, was injected transsclerally into the subretinal space of Long Evans and Wistar rats. After 5 and 12 days respectively the eyes were enucleated and examined by light and electron microscopy. Cryo sections were made of eyes 5 days after MB injection for the application of immunohistochemical techniques using markers for epithelial cells (cytokeratin) and macrophages (ED 1).
Retina, choroid and sclera were not altered in their morphology in the circumference of the MB-containing bubble generated by subretinal injection. After both 5 and 12 days no injected material was found extracellularly in the subretinal space. Especially high amounts of MB were found, in particular 5 days after injection, in lysosomes and melanosomes of RPE cells as well as in cells between choroidal melanocytes. Cells containing MB were seen in contact with choroidal and scleral blood vessels. These MB-containing cells in the choroid and in the sclera were positive for macrophage antibodies.
Subretinal injection was confirmed as a suitable method for placing fluids into the subretinal space without affecting the morphology of the retina. Subretinal injected material was shown to be incorporated into lysosomes and melanosomes of RPE cells. The injected material was subsequently transported through Bruch's membrane to be finally removed from the eye via choroidal and scleral veins, the process involving macrophages.
研究了注入大鼠视网膜下间隙的不可消化物质的命运。
将不能在溶酶体区室中被消化的无毒染料蒙纳斯蓝（MB）经巩膜注射到长 Evans 大鼠和 Wistar 大鼠的视网膜下间隙。分别在 5 天和 12 天后摘除眼球，通过光镜和电镜检查。在注射 MB 后 5 天制作眼球的冷冻切片，用于应用免疫组织化学技术，使用上皮细胞（细胞角蛋白）和巨噬细胞（ED1）的标记物。
视网膜下注射产生的含 MB 气泡周围的视网膜、脉络膜和巩膜形态未改变。在 5 天和 12 天后，视网膜下间隙均未在细胞外发现注射物质。特别是在注射后 5 天，在视网膜色素上皮（RPE）细胞的溶酶体和黑素体以及脉络膜黑素细胞之间的细胞中发现了大量的 MB。可见含 MB 的细胞与脉络膜和巩膜血管接触。脉络膜和巩膜中这些含 MB 的细胞对巨噬细胞抗体呈阳性。
视网膜下注射被确认为将液体注入视网膜下间隙而不影响视网膜形态的合适方法。视网膜下注射的物质被证明可被 RPE 细胞的溶酶体和黑素体摄取。注射物质随后通过布鲁赫膜转运，最终通过脉络膜和巩膜静脉从眼内清除，这一过程涉及巨噬细胞。
