Yao F S, Caserta M T, Wyrwicz A M
Department of Psychiatry and Behavioral Sciences, Northwestern University Medical School, Chicago, IL 60611, USA.
NMR Biomed. 1999 Nov;12(7):463-70. doi: 10.1002/(sici)1099-1492(199911)12:7<463::aid-nbm586>3.0.co;2-s.
We report for the first time in vitro proton and phosphorus NMR spectroscopic analyses of murine brain development from fetal to adult stages. Chloroform-methanol extracts from C57B16/J mouse brain, at ages ranging from 15 days in utero (F15) to adult, permitted the simultaneous investigation of both cytosolic and membrane phospholipid compartments. The protein content of murine brain was determined and used for quantitation of individual metabolite levels. Proton NMR spectroscopy revealed that NAA, considered a neuronal marker, is undetectable at F15. Glutamate, GABA and creatine, however, are present at this time. All four compounds reach maximum levels at 21 days postnatal (P21). Choline and alanine levels are at their peak in fetal brain and progressively fall as the brain develops. Phosphorus NMR spectroscopy shows that phosphatidylcholine, phosphatidylinositol, sphingomyelin, and phosphatidylserine increase steadily from F15 to P21.
我们首次报告了对小鼠从胎儿期到成年期大脑发育的体外质子和磷核磁共振光谱分析。从子宫内15天（F15）到成年期的C57B16/J小鼠脑的氯仿 - 甲醇提取物，允许同时研究胞质和膜磷脂区室。测定了小鼠脑的蛋白质含量，并用于定量单个代谢物水平。质子核磁共振光谱显示，被认为是神经元标志物的NAA在F15时无法检测到。然而，谷氨酸、GABA和肌酸在此时存在。所有这四种化合物在出生后21天（P21）达到最高水平。胆碱和丙氨酸水平在胎儿脑中达到峰值，并随着大脑发育而逐渐下降。磷核磁共振光谱显示，从F15到P21，磷脂酰胆碱、磷脂酰肌醇、鞘磷脂和磷脂酰丝氨酸稳步增加。
