建立一种骨髓增生异常综合征（MDS）/继发性急性髓系白血病来源的T淋巴细胞系K2-MDS。

Establishment of a myelodysplastic syndrome (MDS)/secondary AML-derived T lymphoid cell line K2-MDS.

作者信息

Matsuda M, Maeda Y, Sumimoto Y, Nawata H, Sano T, Higashishiba M, Haga H, Tatsumi Y, Horiuchi F, Irimajiri K, Kanamaru A

机构信息

Third Department of Internal Medicine, School of Medicine, Kinki University, Osaka-Sayama, Osaka, Japan.

出版信息

Leuk Res. 2000 Feb;24(2):103-8. doi: 10.1016/s0145-2126(99)00168-x.

DOI:10.1016/s0145-2126(99)00168-x

PMID:10654444

Abstract

We have established a T lymphoid cell line, K2-MDS, from the peripheral blood mononuclear cells (PBMC) of a patient with acute myeloblastic leukemia (AML) transformed myelodysplastic syndrome (MDS). K2-MDS cells are positive for the expression of CD4, CD5, CD13, CD25, CD71, CD95, HLA-DR and cytoplasmic CD3. Southern blotting analysis shows T cell receptor (TCR) beta chain genes rearrangements, whereas immunoglobulin heavy chain (IgH) genes are not rearranged. Further, the patient PBMC contains TCR beta chain genes rearrangements in the same manner as K2-MDS cells. The data indicate that K2-MDS is a T lymphoid cell line derived from a myelodysplastic clone in the patient PBMC. This new MDS-derived cell line K2-MDS may be a useful in vitro model for studies on the pathogenetic mechanisms leading to MDS.

摘要

我们从一名急性髓细胞白血病（AML）转化型骨髓增生异常综合征（MDS）患者的外周血单个核细胞（PBMC）中建立了一种T淋巴细胞系K2-MDS。K2-MDS细胞表达CD4、CD5、CD13、CD25、CD71、CD95、HLA-DR和细胞质CD3呈阳性。Southern印迹分析显示T细胞受体（TCR）β链基因重排，而免疫球蛋白重链（IgH）基因未重排。此外，患者的PBMC以与K2-MDS细胞相同的方式含有TCRβ链基因重排。数据表明K2-MDS是源自患者PBMC中骨髓增生异常克隆的T淋巴细胞系。这种新的源自MDS的细胞系K2-MDS可能是研究导致MDS发病机制的有用体外模型。