Couper F J, Logan B K
Washington State Toxicology Laboratory, University of Washington and Bureau of Forensic Laboratory Services, Washington State Patrol, Seattle 98134, USA.
J Anal Toxicol. 2000 Jan-Feb;24(1):1-7. doi: 10.1093/jat/24.1.1.
A simple liquid-liquid extraction procedure for the analysis of gamma-hydroxybutyrate (GHB) in biological fluids without conversion to its lactone, gamma-butyrolactone, is described. Following derivatization to its di-TMS derivative, GHB was detected using gas chromatography-electron impact mass spectrometry. Diethylene glycol was used as the internal standard. The limit of quantitation in 1 mL of blood was 1 mg/L, and a linear response was observed over the concentration range 1 to 100 mg/L. Coefficients of variation for both intra-assay precision and interassay reproducibility ranged between 3.9 and 12.0%. GHB was detected in the blood of a sexual assault victim (3.2 mg/L), in the blood of two driving (DUI) cases (33 and 34 mg/L), and in the blood and urine of two nonfatal GHB-overdose cases (blood 130 and 221 mg/L; urine 1.6 and 2.2 g/L). The observed clinical symptoms ranged from confusion, disorientation, vomiting, and nystagmus to ataxia, sinus bradycardia, unconsciousness, and apnea.
描述了一种用于分析生物流体中γ-羟基丁酸（GHB）的简单液-液萃取方法，无需将其转化为内酯γ-丁内酯。将GHB衍生化为其二-TMS衍生物后，使用气相色谱-电子轰击质谱法进行检测。以二甘醇作为内标。1 mL血液中的定量限为1 mg/L，在1至100 mg/L的浓度范围内观察到线性响应。批内精密度和批间重现性的变异系数在3.9%至12.0%之间。在一名性侵犯受害者的血液中检测到GHB（3.2 mg/L），在两起酒后驾车（DUI）案件的血液中检测到（33和34 mg/L），在两起非致命GHB过量案件的血液和尿液中检测到（血液130和221 mg/L；尿液1.6和2.2 g/L）。观察到的临床症状包括意识模糊、定向障碍、呕吐、眼球震颤，以及共济失调、窦性心动过缓、昏迷和呼吸暂停。
