Conte J E, Lin E, Zurlinden E
Department of Epidemiology & Biostatistics, University of California San Francisco 94117, USA.
J Chromatogr Sci. 2000 Jan;38(1):33-7. doi: 10.1093/chromsci/38.1.33.
A technique is presented for the measurement of pyrazinamide in human plasma, bronchoalveolar lavage, and alveolar cells by reversed-phase column chromatography. The assay utilizes acetazolamide as an internal standard, ultraviolet detection at 268 nm, and an acetonitrile-based mobile phase. Preparation of plasma samples requires a simple deproteinization step, resulting in the development of sharp peaks with retention times of 8.4 and 17 minutes for pyrazinamide and acetazolamide, respectively. Bronchoalveolar lavage and alveolar cell suspensions require an acid extraction with ethyl acetate, evaporation to dryness, and reconstitution. This method provides specific, rapid, and reliable determinations of drug concentrations and therefore is suitable for pharmacological studies, particularly those that are designed to quantitate the intrapulmonary concentrations of pyrazinamide.
本文介绍了一种通过反相柱色谱法测定人血浆、支气管肺泡灌洗液和肺泡细胞中吡嗪酰胺的技术。该测定法采用乙酰唑胺作为内标，在268 nm处进行紫外检测，并使用基于乙腈的流动相。血浆样品的制备需要一个简单的脱蛋白步骤，结果是吡嗪酰胺和乙酰唑胺分别在8.4分钟和17分钟的保留时间出现尖锐峰。支气管肺泡灌洗液和肺泡细胞悬液需要用乙酸乙酯进行酸萃取，蒸发至干并复溶。该方法可对药物浓度进行特异性、快速且可靠的测定，因此适用于药理学研究，尤其是旨在定量吡嗪酰胺肺内浓度的研究。
