Matsuda Y
Bull Tokyo Med Dent Univ. 1976 Mar;23(1):27-40.
The distribution of dextran-degrading microorganisms in the saliva and plaque samples from the human oral cavity was assayed on 9 subjects. Approximately 2/3 of the saliva samples degraded Dextran T-150 (Pharmacia, M.W. 150,000) and 1/10 the Blue Dextran (Pharmacia), while 2/5 and 1/8 of the plaque samples degraded Dextran T-150 and Blue Dextran, respectively. Thirty-seven strains of the Blue Dextran degrading bacteria were isolated from the saliva and plaque samples and were classified into 6 groups by their morphology, gram staining and oxygen tolerance. The 24 strains from the 37 isolates, more or less, were shown to degrade the insoluble glucan extracted from Streptococcus mutans FA-1 on the agar plate. The 8 strains, selected from each group, were tested for their activity against the insoluble glucan extracted from Str. mutans (FA-1, HS-6, BHT, CHT, GS-5, LM-7 and PK-1) and Str. salivarius (HHT). The strains belonging to Groups I, II, IV and V showed activity against the insoluble glucan used. Among them, the strains of Group IV (gram negative facultative cocci) and Group V (gram negative strict anaerobic rod) were the most active against the insoluble glucan.
对9名受试者口腔中的唾液和菌斑样本中降解葡聚糖的微生物分布进行了检测。大约2/3的唾液样本能够降解葡聚糖T - 150（Pharmacia公司，分子量150,000），1/10的唾液样本能够降解蓝色葡聚糖（Pharmacia公司），而菌斑样本中分别有2/5和1/8能够降解葡聚糖T - 150和蓝色葡聚糖。从唾液和菌斑样本中分离出37株降解蓝色葡聚糖的细菌，并根据其形态、革兰氏染色和耐氧性分为6组。在这37株分离菌中，有24株或多或少地显示出在琼脂平板上能够降解从变形链球菌FA - 1中提取的不溶性葡聚糖。从每组中挑选出8株菌株，检测它们对从变形链球菌（FA - 1、HS - 6、BHT、CHT、GS - 5、LM - 7和PK - 1）和唾液链球菌（HHT）中提取的不溶性葡聚糖的活性。属于第I、II、IV和V组的菌株对所用的不溶性葡聚糖有活性。其中，第IV组（革兰氏阴性兼性球菌）和第V组（革兰氏阴性严格厌氧杆菌）的菌株对不溶性葡聚糖的活性最强。
