限制性内切核酸酶：其中一个与其他的不同。

Restriction endonucleases: one of these things is not like the others.

作者信息

Galburt E A, Stoddard B L

出版信息

Nat Struct Biol. 2000 Feb;7(2):89-91. doi: 10.1038/72450.

Abstract

The crystal structure of the restriction endonuclease BglII in complex with its DNA target site has been determined. The DNA binding mode and chemistry of catalysis are observed to differ from BamHI which cleaves a similar target site. These observations indicate that more divergence has occurred within this family of proteins than originally thought.

摘要

已确定限制性内切酶BglII与其DNA靶位点复合物的晶体结构。观察到其DNA结合模式和催化化学与切割相似靶位点的BamHI不同。这些观察结果表明，该蛋白质家族内发生的差异比最初认为的更多。