[铜绿假单胞菌外膜蛋白与血浆纤连蛋白的相互作用。细菌黏附素研究]

[Interactions of the Pseudomonas aeruginosa outer membrane proteins with plasma fibronectins. Bacterial adhesin investigation].

作者信息

Rebière-Huët J, Di Martino P, Gallet O, Hulen C

机构信息

Université de Cergy-Pontoise, UFR sciences et techniques, Cergy-Pontoise, France.

出版信息

C R Acad Sci III. 1999 Dec;322(12):1071-80. doi: 10.1016/s0764-4469(99)00106-7.

DOI:10.1016/s0764-4469(99)00106-7

PMID:10656147

Abstract

Pseudomonas aeruginosa adherence is a complex phenomenon largely mediated by pili involving specific receptor-ligand interactions. Anti-fibronectin antibodies as well as plasmatic fibronectin are able to inhibit P. aeruginosa adherence onto A549 cells showing that matricial fibronectin is an actual receptor for this bacterium. Experiments performed in vitro with human plasmatic fibronectin used as receptor and outer membrane proteins of P. aeruginosa as ligands show the presence of four fibronectin-binding proteins. These proteins with molecular mass of 70 +/- 2, 60 +/- 2, 48 +/- 2 and 36 +/- 1 kDa should be adhesins of P. aeruginosa on epithelial cell matrix in a non-pilus mediated adherence.

摘要

铜绿假单胞菌的黏附是一种复杂的现象，主要由菌毛介导，涉及特定的受体-配体相互作用。抗纤连蛋白抗体以及血浆纤连蛋白能够抑制铜绿假单胞菌黏附到A549细胞上，这表明基质纤连蛋白是该细菌的实际受体。用人血浆纤连蛋白作为受体、铜绿假单胞菌外膜蛋白作为配体进行的体外实验表明存在四种纤连蛋白结合蛋白。这些分子量分别为70±2、60±2、48±2和36±1 kDa的蛋白应该是铜绿假单胞菌在上皮细胞基质上非菌毛介导黏附中的黏附素。