用于检测格鲁吉亚共和国长期保存后的产毒白喉棒状杆菌菌株的直接聚合酶链反应
Direct polymerase chain reaction for detection of toxigenic Corynebacterium diphtheriae strains from the Republic of Georgia after prolonged storage.
作者信息
Kobaidze K, Popovic T, Nakao H, Quick L
机构信息
Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Public Health Service, US Department of Health and Human Services, Atlanta, Georgia 30333, USA.
出版信息
J Infect Dis. 2000 Feb;181 Suppl 1:S152-5. doi: 10.1086/315555.
A total of 226 paired nose and throat swab specimens from 113 clinical diphtheria cases from the republic of Georgia were analyzed by direct polymerase chain reaction targeting both A and B subunits of the diphtheria toxin gene, tox. Even after prolonged transport and extensive storage (7-14 months) of the clinical specimens in silica gel packages, direct polymerase chain reaction detected the diphtheria tox gene in 54% of the specimens. Specimens obtained by throat swab were three times more likely than those obtained by nose swab to be positive for Corynebacterium diphtheriae.
对来自格鲁吉亚共和国113例临床白喉病例的226对鼻拭子和咽拭子标本进行了分析，采用针对白喉毒素基因tox的A和B亚基的直接聚合酶链反应。即使临床标本在硅胶包装中经过长时间运输和大量储存（7 - 14个月），直接聚合酶链反应仍在54%的标本中检测到白喉tox基因。通过咽拭子获得的标本检测出白喉棒状杆菌呈阳性的可能性是鼻拭子标本的三倍。
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