Suh H W, Song D K, Huh S O, Kim Y H
Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University, Kangwon-Do, 200-702, S. Korea.
Neuropeptides. 1999 Apr;33(2):101-6. doi: 10.1054/npep.1999.0003.
The effect of total saponin fraction of ginseng injected intrathecally (i.t.) or intracerebroventricularly (i.c.v.) on the antinociception induced by beta-endorphin administered i.c.v. was studied in ICR mice in the present study. The antinociception was assessed by the tail-flick test. Total saponin fraction at doses 0.1 to 1.0 microgram, which administered i.t. alone did not affect the latencies of tail-flick threshold, attenuated dose-dependently the inhibition of the tail-flick response induced by i.c.v. administered beta-endorphin (1 microgram). However, total saponin fraction at doses 1 to 20 microgram, which administered i.c.v. alone did not affect the latencies of the tail-flick response, did not affect i.c.v. administered beta-endorphiun (1 microgram)-induced antinociception. The duration of antagonistic action of total saponin fraction against beta-endorphin-induced antinociception lasted at least for 6 h. Various doses (from 0.1 to 1 microgram) of ginsenoside R(c), but not R(b2), R(d), Rg(1), R(b1)and R(e)injected i.t. dose-dependently attenuated antinociception induced by beta-endorphin administered i.c.v. Our results indicate that total saponin fraction injected spinally appears to have antagonistic action against the antinociception induced by supraspinally applied beta-endorphin. Ginsenoside R(c)appears to be responsible for blocking i.c.v. administered beta-endorphin-induced antinociception. On the other hand, total ginseng fraction, at supraspinal sites, may not exert an antagonistic action against the antinociception induced by supraspinally administered beta-endorphin.
本研究在ICR小鼠中，研究了鞘内（i.t.）或脑室内（i.c.v.）注射人参总皂苷组分对脑室内注射β-内啡肽诱导的镇痛作用的影响。通过甩尾试验评估镇痛作用。单独鞘内注射剂量为0.1至1.0微克的人参总皂苷组分不影响甩尾阈值潜伏期，却能剂量依赖性地减弱脑室内注射β-内啡肽（1微克）诱导的甩尾反应抑制。然而，单独脑室内注射剂量为1至20微克的人参总皂苷组分不影响甩尾反应潜伏期，也不影响脑室内注射β-内啡肽（1微克）诱导的镇痛作用。人参总皂苷组分对β-内啡肽诱导的镇痛作用的拮抗作用持续时间至少为6小时。鞘内注射不同剂量（0.1至1微克）的人参皂苷R(c)，而非R(b2)、R(d)、Rg(1)、R(b1)和R(e)，能剂量依赖性地减弱脑室内注射β-内啡肽诱导的镇痛作用。我们的结果表明，脊髓注射人参总皂苷组分似乎对脊髓上应用β-内啡肽诱导的镇痛作用具有拮抗作用。人参皂苷R(c)似乎是阻断脑室内注射β-内啡肽诱导的镇痛作用的原因。另一方面，在脊髓上部位，人参总皂苷组分可能不会对脊髓上应用β-内啡肽诱导的镇痛作用产生拮抗作用。
