Takeda K, Oshima H, Hayakawa Y, Akiba H, Atsuta M, Kobata T, Kobayashi K, Ito M, Yagita H, Okumura K
Department of Immunology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan.
J Immunol. 2000 Feb 15;164(4):1741-5. doi: 10.4049/jimmunol.164.4.1741.
CD27, a member of the TNF receptor superfamily, has been implicated in T cell activation, T cell development, and T cell-dependent Ab production by B cells. In the present study we examined the expression and function of CD27 on murine NK cells. Murine NK cells constitutively expressed CD27 on their surface. Stimulation with immobilized anti-CD27 mAb or murine CD27 ligand (CD70) transfectans solely could induce proliferation and IFN-gamma production of freshly isolated NK cells and enhanced the proliferation and IFN-gamma production of anti-NK1.1-sutimulated NK cells. Although NK cell cytotoxicity was not triggered by anti-CD27 mAb or against CD70 transfectants, prestimulation via CD27 enhanced the cytotoxic activity of NK cells in an IFN-gamma-dependent manner. These results suggest that CD27-mediated activation may be involved in the NK cell-mediated innate immunity against virus-infected or transformed cells expressing CD70.
CD27是肿瘤坏死因子受体超家族的成员之一，与T细胞活化、T细胞发育以及B细胞依赖T细胞的抗体产生有关。在本研究中，我们检测了CD27在小鼠自然杀伤细胞（NK细胞）上的表达及功能。小鼠NK细胞在其表面组成性表达CD27。单独用固定化抗CD27单克隆抗体或小鼠CD27配体（CD70）转染细胞刺激，可诱导新鲜分离的NK细胞增殖并产生γ干扰素，增强抗NK1.1刺激的NK细胞的增殖及γ干扰素产生。虽然抗CD27单克隆抗体或抗CD70转染细胞未触发NK细胞的细胞毒性，但通过CD27进行预刺激以γ干扰素依赖的方式增强了NK细胞的细胞毒性活性。这些结果表明，CD27介导的活化可能参与NK细胞介导的针对表达CD70的病毒感染或转化细胞的天然免疫。
