Anderson K L, Perkin H, Surh C D, Venturini S, Maki R A, Torbett B E
Department of Molecular Medicine, Scripps Research Institute, La Jolla, CA 92037, USA.
J Immunol. 2000 Feb 15;164(4):1855-61. doi: 10.4049/jimmunol.164.4.1855.
Dendritic cells (DCs) are a heterogeneous population of cells that are specialized for Ag processing and presentation. These cells are believed to derive from both myeloid- and lymphoid-committed precursors. Normal human PBMC-derived, human CD14+ cell (monocyte)-derived, and mouse hematopoietic progenitor-derived DCs were shown to express the hematopoietic cell-restricted, ets family transcription factor PU.1. These populations represent myeloid progenitor-derived DCs. Hematopoietic progenitor cells from PU.1 gene-disrupted (null) mice were unable to generate MHC class IIhigh, CD11c+ myeloid-derived DCs in vitro. Mouse thymic DCs are proposed to be derived from a committed lymphoid progenitor cell that can give rise to T cells as well as DCs. Previously, we showed that CD4 and CD8 T cells developed in PU.1 null mice in a delayed manner and in reduced number. We examined the thymus of 10- to 12-day-old PU.1 null mice and found no evidence of DEC-205+, MIDC-8+ DCs in this tissue. Our findings indicate that PU.1 regulates the development of both thymic and myeloid progenitor-derived populations of DCs, and expand its known role in hematopoietic development.
树突状细胞（DCs）是一类异质性细胞群体，专门负责抗原处理和呈递。这些细胞被认为源自髓系和淋巴系定向祖细胞。已证实，正常人类外周血单核细胞来源的、人类CD14 +细胞（单核细胞）来源的以及小鼠造血祖细胞来源的DCs均表达造血细胞限制性ets家族转录因子PU.1。这些细胞群体代表髓系祖细胞来源的DCs。来自PU.1基因敲除（无效）小鼠的造血祖细胞在体外无法生成MHC II类高表达、CD11c +髓系来源的DCs。有人提出，小鼠胸腺DCs源自一种定向淋巴祖细胞，该祖细胞可产生T细胞以及DCs。此前，我们发现PU.1无效小鼠中的CD4和CD8 T细胞发育延迟且数量减少。我们检查了10至12日龄PU.1无效小鼠的胸腺，未发现该组织中有DEC - 205 +、MIDC - 8 + DCs的迹象。我们的研究结果表明，PU.1调节胸腺和髓系祖细胞来源的DCs群体的发育，并扩展了其在造血发育中的已知作用。
