de la Taille A, Salomon L, Colombel M, Abbou C C, Chopin D, Groux-Muscatelli B
Département d'Urologie, CHU Henri Mondor, Créteil, France.
Prog Urol. 1999 Dec;9(6):1084-9.
The detection of circulating prostatic cells by molecular biology techniques (RT-PCR) can be useful in the staging of localized prostate cancer prior to radical prostatectomy in some institutions. After describing their technique, the authors report their results.
80 RT-PCR were performed: 32 in a control group (including 11 women free of any neoplastic disease, 11 healthy men, and 10 men with benign prostatic hyperplasia before resection), and 48 in patients with prostate cancer (43 with clinically localized cancer and 5 with metastatic cancer).
In the control group, none of 11 women had a positive RT-PCR, 1 of the healthy men was positive (orchidopexy) and 3 of the 11 patients with benign prostatic hyperplasia were positive, but none of them had tumour on the resection chips. None of the 5 metastatic patients were positive. In the patients treated by radical prostatectomy, no correlation was observed between RT-PCR results, pathological stage, positive resection margin status and laboratory progression after radical prostatectomy.
This PSA RT-PCR technique developed in this institution does not appear to be useful for the molecular staging of prostate cancer. This study demonstrates the difficulty of standardization of this technique which limits its routine use.
在一些机构中，通过分子生物学技术（逆转录聚合酶链反应，RT-PCR）检测循环前列腺细胞，对于在根治性前列腺切除术之前对局限性前列腺癌进行分期可能有用。在描述了他们的技术后，作者报告了他们的结果。
共进行了80次RT-PCR检测：32次在对照组（包括11名无任何肿瘤疾病的女性、11名健康男性以及10名切除术前患有良性前列腺增生的男性），48次在前列腺癌患者中（43例临床局限性癌症患者和5例转移性癌症患者）。
在对照组中，11名女性均RT-PCR检测阴性，1名健康男性检测阳性（睾丸固定术），11例良性前列腺增生患者中有3例检测阳性，但他们的切除组织切片中均无肿瘤。5例转移性患者均检测阴性。在接受根治性前列腺切除术的患者中，RT-PCR结果、病理分期、切除边缘阳性状态与根治性前列腺切除术后的实验室进展之间未观察到相关性。
该机构开发的这种前列腺特异性抗原RT-PCR技术似乎对前列腺癌的分子分期无用。本研究证明了该技术标准化的困难，这限制了其常规应用。
