使用基于时间分辨发光的氧传感器测量大鼠脑急性缺氧期间的动脉、静脉和间质氧分压。

Measurement of arterial, venous, and interstitial pO2 during acute hypoxia in rat brain using a time-resolved luminescence-based oxygen sensor.

作者信息

Dunn J F, Nwaigwe C I, Roche M

机构信息

Biomedical NMR Research Center, Hanover, New Hampshire, USA.

出版信息

Adv Exp Med Biol. 1999;471:43-8. doi: 10.1007/978-1-4615-4717-4_6.

DOI:10.1007/978-1-4615-4717-4_6

PMID:10659130

Abstract

This is the first publication using a fiber optic "optode" and a luminescence based pO2 detection method for assessing neural tissue oxygenation. The system was used to simultaneously monitor pO2 in tissue (PtO2) and venous blood (PvO2) during normoxia, hyperoxia and hypoxia. PaO2 was varied by changing inspired oxygen (FiO2) from 0.3 to 0.13. Tissue and arterial pO2 were measured in 5 rats while the simultaneous venous measurements were undertaken in 3 animals. The PtO2 was 29 +/- 10 at an arterial pO2 of 116 +/- 10 (mean +/- SE, n = 5). The PvO2 was consistently higher than PtO2 although PvO2 approached PtO2 as PaO2 declined to 50 mmHg and was lower than tissue pO2 during the complete hypoxic period in one animal. These data indicate that brain venous pO2 is not representative of brain tissue pO2 and support published models predicting that in brain PvO2 is higher than PtO2.

摘要

这是首次使用光纤“光极”和基于发光的pO₂检测方法来评估神经组织氧合的出版物。该系统用于在常氧、高氧和低氧期间同时监测组织中的pO₂（PtO₂）和静脉血中的pO₂（PvO₂）。通过将吸入氧（FiO₂）从0.3改变为0.13来改变动脉血氧分压（PaO₂）。在5只大鼠中测量组织和动脉pO₂，同时在3只动物中进行静脉测量。在动脉pO₂为116±10（平均值±标准误，n = 5）时，PtO₂为29±10。尽管当PaO₂降至50 mmHg时PvO₂接近PtO₂，并且在一只动物的完全缺氧期PvO₂低于组织pO₂，但PvO₂始终高于PtO₂。这些数据表明脑静脉pO₂不能代表脑组织pO₂，并支持已发表的模型预测，即脑中PvO₂高于PtO₂。