Guo Z X, Shen H X
Department of Chemistry, Nankai University, Tianjin, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 1999 Dec;55A(14):2919-25. doi: 10.1016/s1386-1425(99)00168-7.
At pH 2.8 and in the presence of 0.090% p-octylpolyethyleneglycol phenylether, the resonance light-scattering (RLS) spectrum of molybdenum(VI) complex with dibromohydroxyphenylfluorone (DBHPF) has a sharp peak at 586 nm. If the micro protein coexists with Mo(VI) and DBHPF, the RLS intensity of the complex at 586 nm is significantly enhanced by protein due to the binding interaction between protein and DBHPF-Mo(VI) complex. Based on this a new assay for protein is described. The dynamic ranges for bovine and human serum albumins are both 0.05-0.75 mg l-1 with detection limits of 13 and 15 ng ml-1, respectively. Besides high sensitivity, the method is characterized by good reproducibility, rapidity of reaction, good stability of chemical system, commonality of spectrofluorometer, few coexisting substances, especially detergents. The determinations of diluted human serum and urine by this method give the results very close to these by the Coomassie brilliant blue G-250 colorimetry, with relative standard deviations of five duplicates of 1.8-2.5%.
在pH 2.8且存在0.090%对辛基聚乙二醇苯基醚的条件下，钼（VI）与二溴羟基苯基荧光酮（DBHPF）形成的配合物的共振光散射（RLS）光谱在586 nm处有一个尖锐峰。如果微量蛋白质与钼（VI）和DBHPF共存，由于蛋白质与DBHPF - 钼（VI）配合物之间的结合相互作用，蛋白质会显著增强该配合物在586 nm处的RLS强度。基于此，描述了一种新的蛋白质测定方法。牛血清白蛋白和人血清白蛋白的动态范围均为0.05 - 0.75 mg l⁻¹，检测限分别为13和15 ng ml⁻¹。该方法除具有高灵敏度外，还具有重现性好、反应快速、化学体系稳定性好、荧光分光光度计通用性强、共存物质少尤其是去污剂干扰少等特点。用该方法测定稀释的人血清和尿液，结果与考马斯亮蓝G - 250比色法非常接近，五次平行测定的相对标准偏差为1.8 - 2.5%。
Zotero 插件